Western problems

kurzbw at YORK.UCHSC.EDU kurzbw at YORK.UCHSC.EDU
Fri Nov 13 15:43:38 EST 1998


Here are my shares of Western questions:
1.  I am using PVDF membranes for Westerns.  After ECL, I have very high
background with "negative" bands where the proteins were visualized with
previous 20% methanol staining.
2.  How many times can PVDF membranes be stripped?  And after stripping,
can proteins still be visualized with 20% methanol staining?
3.  What are the real life pros and cons of PVDF vs. nitrocellulose?
Thanks.




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