Could anyone tell me where I could purchase an E. coli vector with a temperature sensitive origin of replication (i.e. non-permissive above 42 degrees C)? -- Jeff Terry University of Victoria Department of Biochemistry and Microbiology PO Box 3055 Victoria, BC, Canada V8W 3P6 tel:(250)721-7070 fax:(250)721-8855 terryj at uvic.ca