PCR - Master Mix

Caddie caddie at iname.net
Sun Nov 15 17:04:23 EST 1998


Simply make your own master mix by mixing a large prep of buffer, water,
MgCl2, and primer(s).  Then when you are ready to PCR, aliquot out what you
need, mix in the appropriate polymerase, and add to your samples.

Caddie

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beakerhead at hotmail.com wrote in message <72n9st$otv$1 at nnrp1.dejanews.com>...
>Hi!  I'm looking for a master mix for my PCR reactions. I'd love to just
put
>in my template and primers and not have to do all the pipetting of other
>reagents.  Does anyone have any experience with any products out there?
>Please send me any info-experiences that you might have.
>
>Thanks
>beakerhead at hotmail.com
>
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