Validaton of RNA integraty
Mr. M.J. Lush
mlush at hgmp.mrc.ac.uk
Mon Nov 16 11:01:53 EST 1998
Were producing total RNA to generate a cDNA library
(we have too little tissue to extract mRNA:-(.
Do we need to do a Northern blot to demonstrate that the
RNA is intact or is visualizing the 18S and 28S rRNA bands on an
EtBr/agarose gel good enough evidence that that batch of RNA OK?
TIA
--
Michael
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
NPC rights activist | Nameless Abominations are people too.
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