Does anyone has a protocol for extracting nuclear extract from a piece of frozen tissue for gel shift assay? Thanks in advance Maty Tzukerman Maty Tzukerman, Ph.D. Department of Molecular Medicine B. Rappaport Faculty of Medicine P. O. B. 9649, Bat Galim Haifa 31096, Israel Tel: +972-4-829 52 77/81 Fax: +972-4-851 23 80 e-mail: BIMATY at TX.TECHNION.AC.IL