conversion of lambda ZAP library

Denis Dupuy denis.dupuy at pmtg.u-bordeaux2.fr
Tue Nov 17 13:02:21 EST 1998



mkbarnhart at uh.edu wrote:

> Certainly you can do the conversion all liquid.  Instead of plating the cells
> that were infected with helper phage, try growing them overnight in broth plus
> ampicillin.  If you want, you can scale up appropriately and do a large scale
> DNA prep to get a mixed population of plasmids.
>
> I'm curious why you want to do this.  If you let the group know what you are
> trying to accomplish, we might be able to help more.

I aim to perform a cloncapture using Clontech's new kit . This use the RecA
system to enrich the double strand plasmid population with cDNA homologous of a
sequence of mine.

> On your complexity question, I'm not sure, but if I remember right you always
> lose complexity when you amplify a library.  The conversion will probably do
> the same thing.
>
> Michael Barnhart
> Laboratory Supervisor,
> Connective Tissue Physiology Lab
> University of Houston
> Houston, Texas USA
>
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