AMV vs. MMLV reverse transcriptase

Marc Gregory Busch mgbusch at ucdavis.edu
Wed Nov 18 01:12:01 EST 1998


    I am currently trying to made a cDNA library of total RNA using random
hexamer primers. The old protocol uses MMLV. However AMV reverse
transcriptase is cheaper. Is there a difference and what's better for
certain conditions. After looking the most I could find is that AMV is
stable at a slightly higher temperature. Thank you an advance for any
assistance you may have in the manner.
                                                                    Marc
Gregory Busch

mgbusch at ucdavis.edu





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