Western Blot

Lawrence C. Chen g2bfunky at nospam
Tue Nov 17 14:07:10 EST 1998


Can cell lysate containing guanidinium isothiocyanate (GITC) and
ethanol be analyzed by immunoblotting?

Cell or tissue was lysed with buffer containing GITC and
2-mercaptoethanol.  Ethanol was then added to precipitate the RNA.  I
would like to analysis the protein contents in the supernatant by
immunoblotting for specific antigen.  Should the supernatant be
processed to remove GITC and ethanol prior to western blotting?

Lawrence

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