ss RNA ligase

Vladimir Svetlov svetlov at oncology.wisc.edu
Tue Nov 17 13:59:23 EST 1998


In article <36518DCA.690B3BAD at gengenp.rug.ac.be>, Geert De Jaeger
<gejae at gengenp.rug.ac.be> wrote:

> Does anybody know about the excistence and perhaps availability of
> single strand specific RNA ligase?
> Thanks in advance,

It depends on how you define "specific". T4 RNA ligase - available widely
(Boehringer 799 009) - is ssRNA-"specific", although it would also work on
ssDNA and mono- and diphospates. In complex mixes it will preferentially
ligate ssRNA but mix composition and reaction conditions would determine
the amount of "non-[ssRNA]specific" products (adducts). In other words,
depending on what you are going to use it for you might be able to use
something as simple as T4 RNA ligase. But I can't recall any available
enzyme that would have exclusive ssRNA specificity.

Cheers,
V.

-- 
Vladimir Svetlov, PhD
McArdle Lab for Cancer Res.
UW-Madison
1400 University Ave.
Madison, WI 53706



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