Concentration Hygromycin for HEK-293 cells?

Jarkko Kortesmaa look at signature.below
Fri Nov 20 03:38:49 EST 1998


Tom Vink wrote in message <3653f179.9317838 at news.cc.ruu.nl>...
>Hi,
>
>I am stably transfecting HEK-293 cells with ahygromycin selection
>vector (pCEP4). Anybody knows what concentration hygromycin is
>suitable for theses cells ?
>
>Greetings Tom

I'm using 200 microg/ml, vector is pcDNA3.1/hygro and the hygromycin is from
Invitrogen. A kill curve is a good idea, but I hope this will give you some
idea where to start.

Jarkko Kortesmaa






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