Transformation of cDNA library

Sharron Bannan s.bannan at vetpath.usyd.edu.au
Sat Nov 21 01:52:05 EST 1998


Hi,

I am attempting to electroporate a 'rescued' lambda ZAP II cDNA library (ie.
is in pBluescript)
into E. coli cells (more specifically, XL-1 Blue cells). The problem is that
I get very few colonies
from this (my positive control works well and my cells are competent). I'm
presuming the
problem is that most of the DNA is quite large. Is this the most likely
reason? Is there a better
method?

Thanks in advance,

Sharron Bannan
Dept Veterinary Anatomy and Pathology
University of Sydney NSW 2006
Australia





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