Problem on transformation

John Lum johshi at hotmail.com
Sat Nov 21 00:02:39 EST 1998


Hi,

Bernard P. Murray, PhD wrote:

    Talking about this rearrangement incident, I've recently cloned the
promotor region of a gene. After transforming back to XL-1 Blue, I got clones
of various length and even with different arrangement of restriction sites.
Is it related to the rearrangement incident..?? I haven't got the sequence
yet so I dunno if there are any inverted repeat or strange sequence in it.

    One of my friend suggest me to switch the host to SRB...do anyone got any
information about it..??

 Thanks in advance..!!

Regards,
John Lum




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