Problem on transformation

Rich Dudley rdudley+ at pitt.edu
Fri Nov 20 15:45:28 EST 1998


Long-Cheng li wrote:

> Hi, Everybody:
>
> Recently I got a strange problem trying to clone a promoter sequence
> into pGL3 enhancer. After transformation, we could get positive  clone
> as tested by PCR of the minipreps. The problem was the positive clone
> became negative after maxiprep either tested by PCR or digestion. This
> phenomona repeated itself several times. If anybody has any suggestion,
> please give me a reply. Thanks.
>
> Long
>
> longli at itsa.ucsf.edu

Check your PCR reagents.  Sounds like they may be contaminated.

rich

--- --- --- -- -- -- --- --- ---
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
---> search BIONET archives at http://www.bio.net <---





More information about the Methods mailing list