fragment isolation problem

Marcus Wernitz wernitz at landw.uni-halle.de
Sun Nov 22 07:22:38 EST 1998


Try a 'dirty' cloning: Don't try to seperate your fragments on gel and loose al
lot while cleaning, instead use all to ligate and transform. After blue/white
selection do a PCR with the plasmid primer to find out, which colony got the
right insert.


Francisco Villamon schrieb:
> 
> Hi all!
> I want to isolate one fragment to cloning.....the problem is the
> separation of  one fragment about 2.4 kb from another one about 2.9 kb
> in an agarose gel. The difference beetwen both after running is so short
> making difficult the isolation by gel cutting.
> What can I do for separate more the fragments?
> Thanks in advanced,
> 
> Francisco Villamon Cifuentes
> E-mail addresses: fvillamon at hotmail.com
>                   f.villamon at mailexcite.com
> Since August, 1998
> 
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