fragment isolation problem

Chris Boyd chrisb at
Mon Nov 23 05:05:11 EST 1998

Francisco Villamon (fvillamon at wrote:
: Hi all!
: I want to isolate one fragment to cloning.....the problem is the 
: separation of  one fragment about 2.4 kb from another one about 2.9 kb 
: in an agarose gel. The difference beetwen both after running is so short 
: making difficult the isolation by gel cutting.

You've got to be joking.  That's a 20% difference in size, which for
that size range means the fragments are quite easily resolvable on a
0.8% gel. All you need do is run the gel further -- say until the blue
dye is 10 cm or so from the wells.

Best wishes,
Chris Boyd                      | from (but not \  MRC Human Genetics Unit
Christopher.Boyd at  | on behalf of) /      Crewe Rd, Edinburgh          EH4 2XU, SCOTLAND

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