fragment isolation problem
chrisb at hgu.mrc.ac.uk
Mon Nov 23 05:05:11 EST 1998
Francisco Villamon (fvillamon at hotmail.com) wrote:
: Hi all!
: I want to isolate one fragment to cloning.....the problem is the
: separation of one fragment about 2.4 kb from another one about 2.9 kb
: in an agarose gel. The difference beetwen both after running is so short
: making difficult the isolation by gel cutting.
You've got to be joking. That's a 20% difference in size, which for
that size range means the fragments are quite easily resolvable on a
0.8% gel. All you need do is run the gel further -- say until the blue
dye is 10 cm or so from the wells.
Chris Boyd | from (but not \ MRC Human Genetics Unit
Christopher.Boyd at hgu.mrc.ac.uk | on behalf of) / Crewe Rd, Edinburgh
http://www.hgu.mrc.ac.uk/Users/Christopher.Boyd EH4 2XU, SCOTLAND
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