AFLP problem

[ummorie1 at cc.UManitoba.CA]

Hello:
	I am using AFLP protocol to locate molecular markers.   It seems 
that each time I run a gel about one third of my gel consists of  blank 
lanes.  These blank lanes seem to be random and unrepeatable. I've already
tried changing my DNA samples and reagents. I was wondering if 
anyone has come across this phenomenon before and/or have any suggestions 
for me. 
Thanks a bunch.

Larisa Morier
Master's student
Departement of Plant Science
University of Manitoba

email: ummorie1 at cc.umanitoba.ca