Low Efficiency from Electroporation
campbesg at tcd.ie
Mon Nov 23 08:05:44 EST 1998
I have been having problems transforming my ligation product into a
competent XL1blue strain. First of all I am only getting very few colonies
and secondly when I try to retrieve the plasmid I and getting a smear on
the gel as if the plasmid has somehow degraded.
Has anybody experienced anything similar to this,
Thanks in advance.
Moyne Institute of Preventive Medicine,
Trinity College Dublin,
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