pGAD primers
Sailesh Surapureddi
Saisu at mcb.liu.se
Mon Nov 23 09:03:52 EST 1998
>Could any two hybrid users out there tell me the sequences for suitable
>PCR or sequencing primers (forward and reverse) for the pGAD series of
>plasmids
>
>Thanks
Here is the primer sequence info.
5'taccactacaatggatg3'
I have been using the above clontech's suggested sequencing primer and the
reverse sequence was designed to our sp. sequence.
Sometime back I remember that the Clontech guys have also sent me the
amplification primer seq. if you want that, I can post it for you.
Sailesh
Sailesh Surapureddi, Ph.D
Communicators
Plan #12, Cellbiologi Work +46-13-223917
Dept. of Biomedicine & Surgery Res +46-13-274718
Linköpings University Cel +46-70-4916570
S 581 85, Linkoping Fax +46-13-224149
Sweden. Email Saisu at mcb.liu.se
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