HELP WHIT SOUTHERN WHIT SEMI DRY SYSTEM!!!!!
svetlov at oncology.wisc.edu
Tue Nov 24 14:03:39 EST 1998
In article <73el7m$r5t$1 at nnrp1.dejanews.com>, gigles at fvet.uba.ar wrote:
: I know about the semi dry system for protein-transfer, but I don't know how
: can I do it with DNA. I need to know the volts, amp, time and the buffers
: that I must use. Thanks.
Would you please calm down? It is no less than a fourth posting with or
regarding a trivial question the answer to which can be found in
appropriate user manual for Pharmacia Multiphor/Novablot, BioRad Transblot
or whatever unit you have. Electroblotting from DNA PAGE and agarose gels
is routinely done using diluted running buffer (0.3-0.5XTBE/TAE) at 30-40 V
(100-125 mA), time depends on the gel. The electric parameters here are
only ball park numbers and have to be determined more precisely using
manufacturer's recommendations for a given model (or a model with
comparable geometry and materials).
V. Svetlov, Ph. D.
McArdle Laboratory for Cancer Research
University of WI, Madison
1400 University Ave.
Madison, WI 53706
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