Myc, His or other tags

John Thompson joth at uni-freiburg.de
Tue Nov 24 09:54:06 EST 1998


Has anyone already tried to use vectors encoding more than one tag that are
directly next to each other in an ELISA test. Specifically, we wish to bind
a pSecTag vector-encoded protein to nickel plates via the His tag and
confirm binding using an anti-myc antibody. As these two tags are only
separated by four or five amino acids, we are not certain if the myc tag
will be "visible" to the anti-myc antibody. We would be grateful for any
infos.

John Thompson
Institute of Immunobiology
University of Freiburg
Germany





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