RNA-RNA hybrid denaturation

Pascal Mertens Pascal.Mertens at fundp.ac.be
Tue Nov 24 08:51:01 EST 1998

I didn't find any info about that in the archives so I guess it's not
redundant with any previous message:

Does anybody know about a method(protocol) to denature RNA-RNA hybrids on
a nylon membrane before probe hybridization? The hybrids cannot be
denatured before beeing lifted on the membrane (these are dsRNA viral
Thanks in advance for any response

Pascal Mertens
Laboratoire d'Immunologie et Microbiologie
61 Rue de Bruxelles
5000 Namur, BELGIUM
Phone: 32 81 724438
Fax: 32 81 724420

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