RNA-RNA hybrid denaturation

Pascal Mertens Pascal.Mertens at fundp.ac.be
Tue Nov 24 08:51:01 EST 1998


I didn't find any info about that in the archives so I guess it's not
redundant with any previous message:

Does anybody know about a method(protocol) to denature RNA-RNA hybrids on
a nylon membrane before probe hybridization? The hybrids cannot be
denatured before beeing lifted on the membrane (these are dsRNA viral
plaques).
Thanks in advance for any response
Pascal

-- 
Pascal Mertens
Laboratoire d'Immunologie et Microbiologie
URBM-FUNDP
61 Rue de Bruxelles
5000 Namur, BELGIUM
Phone: 32 81 724438
Fax: 32 81 724420



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