Protein SDS-PAGE

brett at BORCIM.WUSTL.EDU brett at BORCIM.WUSTL.EDU
Tue Nov 24 22:03:38 EST 1998


>hi, everyone, i have a question about Protein SDS-PAGE. i run the gel which
>is 0.75mm thickness, 2cm height of stacking get plus 12cm height of
>separating gel under constant current of 30mA for two gels(15mA for each).
>it runs so slowly that takes about more than 10 hours to run to the bottom.
>the time is much longer than that in my lab's protocol. the concentration of
>the stacking gel and separating gel are 3% and 10% respectively. i am sure
>that there is no error in my all buffer. is there anyone who has experience
>in it can give me some advice about why it is so slow and what i should
>check about it. how long do you spend to run the gel like this? thank you
>for your help and advice.

It sounds like you may indeed have an improperly made buffer.  How do you
know "there is no error"?  I'd borrow a lab-mates reagents to troubleshoot
this or maybe even just start all over.




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