help with heteroduplex analysiss

[Paul Wakenight]

I am trying to resolve 100-290 bp fragments (not together) from a
mouse/hamster panel. I have seen many types of gel concentrations/conditions
etc.  Does anyone have practical advice as to running this on one of the
minislabs?  also, at what point would you consider a mutation non-detectable
and move on to new techniques/primers?


thanks


pw at cubsps.bio.columbia.edu