help with heteroduplex analysiss

Paul Wakenight pw at cubsps.bio.columbia.edu
Tue Nov 24 17:13:29 EST 1998


I am trying to resolve 100-290 bp fragments (not together) from a
mouse/hamster panel. I have seen many types of gel concentrations/conditions
etc.  Does anyone have practical advice as to running this on one of the
minislabs?  also, at what point would you consider a mutation non-detectable
and move on to new techniques/primers?


thanks


pw at cubsps.bio.columbia.edu   






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