RNA concentration?

Jun Zhang junz at sfu.ca
Thu Nov 26 01:00:33 EST 1998


Hi,

I have a simple question: Can "high" amount RNA (or mRNA), say 1ug/ul, 
inhibit either reverse transcription reaction or PCR in 50ul RT-PCR reaction.

The RNA (or mRNA) is from culture cells isolated with BM or Qiagen RNA 
(or mRNA) isolation kit.

Your input is highly appreciated.

Thanks
Mark 




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