RNA concentration?

Jun Zhang junz at sfu.ca
Thu Nov 26 01:00:33 EST 1998


I have a simple question: Can "high" amount RNA (or mRNA), say 1ug/ul, 
inhibit either reverse transcription reaction or PCR in 50ul RT-PCR reaction.

The RNA (or mRNA) is from culture cells isolated with BM or Qiagen RNA 
(or mRNA) isolation kit.

Your input is highly appreciated.


More information about the Methods mailing list