GFP localization ?

Wolfgang Schechinger Wolfgang.Schechinger at med.uni-tuebingen.de
Fri Nov 27 01:09:54 EST 1998


Hi all, 

When transfecting 293HEK and NIH 3T3 MF-cells with GFP (humanized 
S65T in pCDNA3) I noticed the brightest fluorescence in the nucleus, 
while a (certain) fusion protein doesn't. Is there a reason for this? 
Actually, I had expected that there was no fluorescence in the 
nucleus since there's no nuclear import sequence. Am I wrong? Is 
there resonance energy transfer from DNA to GFP? 
The phenomenon occurs when using a Hg lamp with filters for FITC as 
well as a 488nm argon laser and a confocal microscope.

Any ideas?

Wolfgang
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Wolfgang Schechinger         
University of Tuebingen, Germany
email: wgschech at med.uni-tuebingen.de * wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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