Transfecting primary culture

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Thu Nov 26 20:40:58 EST 1998


In article <73jgei$4u0$1 at unix2.glink.net.hk>, "Andrew Leung" <leungkc at glink.net.hk> wrote:
>Hi all,
>
>    Is there any body having experience in transfecting primary culture with
>plasmid vector? Any advice is greatly appreciated.
>

Depending on the culture origin, you may or may not have good luck
with one if the 1001 lipofection reagents (search the literature!) but
IMHO it's better to simply use electroporation. 

All standard ideas about optimization apply.

        - Dima



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