cDNA library of early meiosis!!

Ryan Whitford rwhitford at waite.adelaide.edu.au
Thu Nov 26 19:53:46 EST 1998


Hi

I am trying to extract mRNA from an extremely small amount of tissue.  Using
wheat anthers undergoing early meiosis to be specific.  This material is
very precious and I would like to know if anyone has a good reliable
protocol for extracting good quality poly A mRNA from this small amount of
tissue.  How much tissue is enough??? Will be used in construction of an EST
database specific for wheat meiosis.

Thanks for you help!!

Ryan Whitford
CRC Molecular Plant Breeding
University of Adelaide





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