SV40 origins and early promoters

Simon Dawson Simon.Dawson at nott.ac.uk
Thu Nov 26 03:48:50 EST 1998


Hi All,
        Does anyone out there know what effect having _two_ SV40 origins/early
promoter regions in the same mammalian expression vector would have if the
vector is transfected into cells....usually cells which don't express SV40
large T?? Or even better, what would happen if the vector went into something
like COS cells? Does replication initiate at two sites and if so, what would
happen??
    Thanks for any help!


            Simon Dawson.



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