Stable transfectants in Jurkat cells
Frank O. Fackelmayer
fof1 at chclu.chemie.uni-konstanz.de
Fri Nov 27 10:27:21 EST 1998
We just did that. The only way that worked in our hand was Qiagen´s
Superfect reagent (no affiliation). It does not give a high number of
transiently transfected cells (only about 1%), but that is no problem
when making stable transfections because you select anyway. All other
Hope this helps,
Oskar Fdz-Capetillo wrote:
> Hi there:
> Has anybody tried to develop stable transformants in Jurkat cells?
> I am trying to do it but it doesn´t work very well ( with
> lipofectamine transfection ).
> Any suggestions?
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