DNA qauntification using fluoremetry

John Thompson jrt at home.com
Sun Nov 29 21:38:07 EST 1998


Greg Douhan <gdouhan at mail.wsu.edu> wrote:

>Does anybody have a good protocol for quantifing DNA using a 96 well
>fluoremeter?

Try SybrGreen or PicoGreen from Molecular Probes.
<http://www.molecularprobes.com/>

Mix the dye in TE with ng levels of DNA and read with fluorescein
filters. SybrGreen is cheaper but RNA interferes to a greater extent
than with PicoGreen.

Standard disclaimers apply.

Regards,
John Thompson
Merck Research Labs



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