DNA qauntification using fluoremetry

[John Thompson]

Greg Douhan <gdouhan at mail.wsu.edu> wrote:

>Does anybody have a good protocol for quantifing DNA using a 96 well
>fluoremeter?

Try SybrGreen or PicoGreen from Molecular Probes.
<http://www.molecularprobes.com/>

Mix the dye in TE with ng levels of DNA and read with fluorescein
filters. SybrGreen is cheaper but RNA interferes to a greater extent
than with PicoGreen.

Standard disclaimers apply.

Regards,
John Thompson
Merck Research Labs