'harmless' PCR test

Bernard P. Murray, PhD bpmurray*STUFFER* at socrates.ucsf.edu
Mon Nov 30 21:59:32 EST 1998

In article <01BE1D39.BAE713C0.MackayJF at bmnz.co.nz>, "MackayJF at bmnz.co.nz"
<MackayJF at bmnz.co.nz> wrote:

> Hi all,
> I am looking for a 'fun' (for want of a better word) PCR test to introduce 
> people to molecular diagnostics. I checked out a physical performance gene 
> (in Nature earlier this year) but checking the references, it looks like a 
> particular genotype from this particular assay is a cardiac risk marker!
> Scratch that one.
> I would be grateful if anyone could suggest a harmless candidate gene and 
> the appropriate reference for primer sequences.....
> Cheers,
> John

How about using one of the primer sets used to determine sex?
I have used Zfx/Zfy, smc-x/smc-y and sry in mice so I assume
there must be equivalent targets in humans.

...or would this have the same problem as a risk marker?

Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA

More information about the Methods mailing list