Gels keep cracking
pxpst2 at unixs.cis.pitt.edu
Mon Nov 30 13:08:27 EST 1998
In article <73ui0k$eer$1 at nnrp1.dejanews.com>,
theodorn at medlib.georgetown.edu wrote:
> It's funny, but when I used to do a lot of metabolic labelling experiments, I
> used good old PPO/DMSO, which seems to be out of fashion these days, but I
> never had a gel break on the dryer (even 15% gels). They seem pretty tough
> after the PPO is precipitated into the gel; you can pretty much throw them
But there is a good reason why this techinique has lost favor. The
chemicals are rather nasty.
" Don't you eat that yellow snow
Watch out where the huskies go"
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