PFGE plugs problem

erlendur at biotek.uio.no erlendur at biotek.uio.no
Thu Oct 1 14:18:23 EST 1998


I am trying to make a PFGE plugs from a strain of gram-positive bacteria.
When I cut the DNA in the plugs with an enzyme that should (have
previously) show bands up to 1300 kb I only get small fragments up to
approx 300 kb.

Does anybody have suggestion what is the problem?

Regards,

Erlendur



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