Separation of ssDNA from dsDNA, but in gels !

Andreas Becker becker at ps1515.chemie.uni-marburg.de
Thu Oct 1 10:56:05 EST 1998


On Tue, 22 Sep 1998 08:57:06 GMT, "Csaba Kiss" <csakis at ki.se> wrote:

>
><j.migeon at cerep.com> wrote in message 6tunbt$3d2$1 at orthanc.reference.com...
>>If anyone knows how to effectively separate single-stranded
>>DNA from double stranded DNA (no gels),  I would be grateful
>>for any suggestions.
>
>Hi!
>You could use hydroxylapatite. It unspecifically binds dsDNA but not ssDNA.
>Do a search in the medline, look for old articles from the '70s and '80s.

Is there an appropriate method to do that for an analytical approach
with a gel. For ssDNA denaturating and for non-denaturating gels are
reommendet. But what to use for the analytics of both?

Thanks for any comments

Andreas Becker





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