proliferation assays

[Heather Medbury]

Hi netters

Has anyone done proliferation assays with adherent cells?
I need to quantify the proliferation of smooth muscle cells, and the
methods in the journal articles are too brief to follow. They involve
TCA precipitation of T* incorporated DNA.
I would be grateful for an outline of this protocol or any other means
of quantifying adherent cell proliferation

Thanks

Heather Medbury
Surgery
Sydney AUS.