proliferation assays

Olivier Gandrillon ogandril at cri.ens-lyon.fr
Fri Oct 2 09:02:22 EST 1998


Hi

You can use the non-radioactive WST-1 assay from Boehringer. It works 
pretty well for me. The protocol is pretty straitforward. Let me know if 
you want more info.

Olivier

Heather Medbury wrote:
> 
> Hi netters
> 
> Has anyone done proliferation assays with adherent cells?
> I need to quantify the proliferation of smooth muscle cells, and the
> methods in the journal articles are too brief to follow. They involve
> TCA precipitation of T* incorporated DNA.
> I would be grateful for an outline of this protocol or any other means
> of quantifying adherent cell proliferation
> 
> Thanks
> 
> Heather Medbury
> Surgery
> Sydney AUS.



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