Sparation of cultured HUVEC using a non-enzymatic method?
crash at mad.scientist.com
Fri Oct 2 04:15:23 EST 1998
I´m not sure if this group is the right one to post this message in, but in
the end I will perform some PCR using these cells and that may qualify my
message for this group.
To the problem.
Does anyone know a method for separation of cultured HUVEC for
flowcytometry? It is importatnt that we don´t damage any adhesion proteins
on the suface. Therefore trypsin may not be an option for us.
Is the only possible solution to use EDTA with mechanical
Or is there other non-enzymatic methods to get single cells?
Clinical Research Center
Dept. of Internal Medicine
Linköping University Hospital
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