shuttle/yeast->bacteria

%REALNAME% Alan.Hair at ncl.ac.uk
Fri Oct 2 10:14:48 EST 1998


I am just about to finish a one-hybrid screen for the first time (and
hopefully the only time). I am the stage where I will isolate DNA from my
yeast positives and use it to transform E.Coli. I am told this is very
inefficient.
Question: What is the current "state of the art" for doing this? Presumably
it involves making hypercompetent bugs and/or optimising the plasmid
recovery from the yeast. Any hints/tips/methods/opinions would be greatly
appreciated.
Cheers
Alan
Alan.Hair at newcastle.ac.uk





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