DNA-isolation from 65 000 blood samples
dkim at NMSU.Edu
Tue Oct 6 12:02:51 EST 1998
I have not personally isolated so many samples, but you would get more
meaningful responses if you give a few details:
-How much DNA do you need from each sample?
-What kind of quality/purity do you want or need? That is, will you be
doing gels and blots from them, or just PCR?
-Do you plan to use robotics?
-Do you need to save some of the blood for an archive storage sample?
-Can you use 96-well plates or other microplate-formatted device?
Ketil Thorstensen <ketilt at dmf.unit.no> wrote:
: I am planning to isolate DNA from 65 000 blod samples. The samples are frozen.
: About half of them are anticoagulated with EDTA whereas the other are clotted.
: The volume of blood is about 5 ml and I intend to use all of it for islation
: of DNA.
: Has anyone performed DNA-isolation from such a large number of samples? How
: did you do it effectively?
: Is there any efficient way to dissolve the blood clots and still be able to
: isolate the DNA?
: I would appreciate all suggestions and ideas on how to handle this.
: Thank you very much.
: Ketil Thorstensen (Ph.D.)
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