three fragment cloning

Joel Baguet Joel.Baguet at
Tue Oct 6 11:54:33 EST 1998

I have done this kind of experiment by adding the three fragments 
together and different ratios between vector and the inserts
and it was OK ... after 48 minipreps there was only 3 

to work quickly you can do PCR directly on the bacteria colonies
or transfer the bacteria on a nitrocellulose membrane followed by a 
screening using a radioactive oligonucleotide probe

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