crystal violet DNA staining
ckafer at iastate.edu
Thu Oct 8 15:15:59 EST 1998
I think she was asking about staining agarose gels and not bacterial
a 1000X sol'n of CV is 10 mg/ml in H2O for this purpose. Methanol is
On Thu, 08 Oct 1998 09:03:56 +0100, see_sig at cmtech.co.delete.uk
(Richard P. Grant) wrote:
>In article <361BC628.612B at calstatela.edu>, Vellanoweth's Lab
><ddeloss at calstatela.edu> wrote:
>: I have a question concerning using crystal violet to visualize DNA.
>: First, I need to know how to dissolve the crystal violet because we have
>: it in powder form. I don't know what solvent to use or in what
>: proportions. Any information is greatly appreciated. Replies can be
>: directly sent to "cgama at calstatela.edu". Thank you very much.
>IIRC, methanol should be used to dissolve CV. You can then use a 10%
>solution in water to stain cells . . .
>Richard P. Grant MA DPhil | rgrant at cmtech.co.uk
>Senior R&D Scientist | work: www.cmtech.co.uk
>Cambridge Molecular | home: www.avnet.co.uk/adastra
> -- 'Practising biochemistry without a licence' - E. Chargaff --
More information about the Methods