crystal violet DNA staining

Biohazard ckafer at iastate.edu
Thu Oct 8 15:15:59 EST 1998


I think she was asking about staining agarose gels and not  bacterial
stains, BTW.  

a 1000X sol'n of CV is 10 mg/ml in H2O for this purpose.  Methanol is
not necessary.


On Thu, 08 Oct 1998 09:03:56 +0100, see_sig at cmtech.co.delete.uk
(Richard P. Grant) wrote:

>In article <361BC628.612B at calstatela.edu>, Vellanoweth's Lab
><ddeloss at calstatela.edu> wrote:
>
>: I have a question concerning using crystal violet to visualize DNA.
>: First, I need to know how to dissolve the crystal violet because we have
>: it in powder form. I don't know what solvent to use or in what
>: proportions. Any information is greatly appreciated. Replies can be
>: directly sent to "cgama at calstatela.edu". Thank you very much.
>
>IIRC, methanol should be used to dissolve CV.  You can then use a 10%
>solution in water to stain cells . . .
>
>-- 
>Richard P. Grant MA DPhil     |             rgrant at cmtech.co.uk
>Senior R&D Scientist          |             work: www.cmtech.co.uk
>Cambridge Molecular           |      home: www.avnet.co.uk/adastra           
> -- 'Practising biochemistry without a licence' - E. Chargaff --




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