Phosphopeptide binding

Rob mbzrl at mbn1.biochem.nottingham.ac.uk
Wed Oct 7 02:43:14 EST 1998


Hi
I'm using an immobilised phosphopeptide (containing phosphoserine) to
affinity-purify a binding protein - the binding stage works fine, and is
phosphoserine-dependent, since the nonphosphorylated peptide does not bind.
Can anyone suggest a method to elute the (native) binding protein, perhaps
something based on 'competition' using a phosphate buffer?
Many thanks in advance
Rob Layfield 



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