Isolation of mRNA for cDNA probe?

ELADER at AMBION.COM ELADER at AMBION.COM
Fri Oct 9 21:14:49 EST 1998


In article <361d38c5.280871845 at news.cc.uic.edu>,
  levenson at uic.edu (Victor Levenson) wrote:
> Hello, nettters:

>  Hence the question(s): what is your experience with cDNA used as a
> probe (especially for differential screening), what procedure do you
> use to eliminate genomic DNA, is Trizol followed by polyA+ isolation
> sufficient in your opinion, and - if you are using a kit - which one
> would you recommend based on price/performance?
>
> Thanks for your input!

In my experience, I think you should dT select the RNA and then dT prime the
RNA in the RT reaction. I don't think the bit of DNA is a problem. Only 2
copies per cell to start with, right?

Eric

elader at ambion.com

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