trouble cloning a G/C-rich fragment

Juergen Brosius brosius at
Sat Oct 10 09:58:20 EST 1998

After mutagenizing a fragment (about 2 kb - several point mutations were
made) of the HERG gene tried to clone it back into original vector
(pSP64/full-length HERG/A-tail of about 30 nt at 3' end of HERG).  While
the original vector appears to be stable we obtained only deletions of
various lengths.  Tried different E. coli strains including SURE.  Any
suggestions would be appreciated.

                                          usual disclaimers
Juergen Brosius
Inst. f. eXp. Pathol.
University of Muenster
Von-Esmarch-Str. 56
D-48149 Muenster, Germany

Fax: +49  251  835 8512
Tel: +49  251  835 8511

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