trouble cloning a G/C-rich fragment
brosius at uni-muenster.de
Sat Oct 10 09:58:20 EST 1998
After mutagenizing a fragment (about 2 kb - several point mutations were
made) of the HERG gene tried to clone it back into original vector
(pSP64/full-length HERG/A-tail of about 30 nt at 3' end of HERG). While
the original vector appears to be stable we obtained only deletions of
various lengths. Tried different E. coli strains including SURE. Any
suggestions would be appreciated.
Inst. f. eXp. Pathol.
University of Muenster
D-48149 Muenster, Germany
Fax: +49 251 835 8512
Tel: +49 251 835 8511
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