Long PCR: How long?

marcv2 at my-dejanews.com marcv2 at my-dejanews.com
Mon Oct 12 12:34:57 EST 1998


> While going through a lot of journals I am trying to optimize the maximum
> length of sequences that can be PCRed but could not find a proper number. Can
> anybody comment on it. Also generally how long sequences are PCRed?
>
> Ravi Gupta.

Hi Ravi,
In the lab, we're abble to amplify up to 15 Kb DNA fragment length, with the
Expand Long template PCR, sold by Boehringer. In this kit, it's possible to
amplify 40Kb DNA fragments !! (I've never tried this, but may be...)

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