Suggest direct PCR-sequencing kit?
jtardiff at westnet.com
Tue Oct 13 14:45:24 EST 1998
My student will be sequencing PCR products amplified from human archival
tissue-derived DNA for mutation analysis. The fragments are 200-300 bp
It's been a *long* time since I've done any manual sequencing and I know
things have improved a bit.
Can anyone suggest a kit or good protocol for direct sequencing of PCR
Pitfalls, warnings, etc are also appreciated.
More information about the Methods