subcellular fractionation protocol and markers, please

Mike Kalchman mike.kalchman at
Tue Oct 13 11:45:02 EST 1998

Hi I am trying to purify sER membranes from brain tissue.  I am getting
golgi contamination.

Can someone give me the following direction, please:

1.  The best way to limit contamination through the gradient

2.  Commercially available golgi and ER markers?

Thanks a ton.

Michael Kalchman, BSc, PhD

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