DNA-isolation from 65 000 blood samples
Patrick F.H. Lai the Graduate Student
pfhlai at LOOKSMART.COM
Tue Oct 13 23:12:40 EST 1998
---- Begin Original Message ----
Ketil Thorstensen <ketilt at dmf.unit.no> wrote:
: I am planning to isolate DNA from 65 000 blod samples. The samples are frozen.
: About half of them are anticoagulated with EDTA whereas the other are clotted.
: The volume of blood is about 5 ml and I intend to use all of it for islation
: of DNA.
: Has anyone performed DNA-isolation from such a large number of samples? How
: did you do it effectively?
: Is there any efficient way to dissolve the blood clots and still be able to
: isolate the DNA?
: I would appreciate all suggestions and ideas on how to handle this.
: Thank you very much.
: Ketil Thorstensen (Ph.D.)
---- End Original Message ----
Dear Dr. Ketil Thorstensen,
I am not too sure if this is what you have in mind, but take a look anyways:
Hope this is useful info. :-)
Patrick F.H. Lai < PFHLai at looksmart.com >
University of Toronto
Toronto, Ontario, Canada
P.S. I am merely a GradStudent.
One should take a HUGE grain of salt with whatever I have typed above.
Good Luck. :-)
or keep looking.
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