chenlinan at 263.NET chenlinan at 263.NET
Tue Oct 13 19:05:51 EST 1998

Hi, all
What is RNA aptamer? 


chen linan

Controlling Gene Expression in Living Cells Through Small Molecule-RNA
Interactions.   Geoffrey Werstuck and Michael R. Green Science 1998 October
9: 296-298.

Short RNA aptamers that specifically bind to a wide variety of ligands in
vitro can be isolated from
randomized pools of RNA. Here it is shown that small molecule aptamers also
bound their ligand in vivo,
enabling development of a method for controlling gene expression in living
cells. Insertion of a small
molecule aptamer into the 5' untranslated region of a messenger RNA allowed
its translation to be
repressible by ligand addition in vitro as well as in mammalian cells. The
ability of small molecules to
control expression of specific genes could facilitate studies in many areas
of biology and medicine. 

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