RNase protection assay
Patrick F.H. Lai the Graduate Student
pfhlai at LOOKSMART.COM
Wed Oct 14 00:36:33 EST 1998
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Does anyone know what a RNase protection assay is and what is its advantage
over filter hybridization in quantitating gene transcription?
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Dear Dan and Netters,
This is how I understand what RPA is, correct me if I am wrong:
RPA is a simple way of detecting and quantitating RNA instead of doing a Northern blot. Basically, a anti-sense probe (typically a few hundred bases long) is mixed in with your sample of RNA extract. Probe binds to your RNA of interest and is protected when you throw in ssRNA-specific RNases.
Run a gel and transfer. If your probe is radiolabelled or fluorescent-tagged, you will see a band on your blot. You design your probes, so you know what size of the band to expect.
Advantage ? One can detect several RNA at the same time.
Simply use probes of different sizes that one can resolve in a gel.
Check out www.ambiom.com
I heard that their newest kit even allows up to 6 RPA in one shot!
BTW, has anyone actually took up Dr. Eric Lader's offer to try out their RNAlater! solution ?
How is it ?
Hope this is useful info. :-)
Patrick F.H. Lai < PFHLai at looksmart.com >
University of Toronto
Toronto, Ontario, Canada
P.S. I am merely a GradStudent.
One should take a HUGE grain of salt with whatever I have typed above.
Good Luck. :-)
or keep looking.
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